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STEMdiff™ Cerebral Organoid Kit
Culture medium kit for establishment and maturation of human cerebral organoids
STEMdiff™ Cerebral Organoid Kit (Catalog #08570) is a defined, serum-free cell culture medium that enables the robust generation of human pluripotent stem cell (hPSC)-derived cerebral organoids in a simple four-stage protocol. Cerebral organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative of the developing human brain. STEMdiff™ Cerebral Organoid Kit has been optimized to increase efficiency and reproducibility of organoid formation based on the formulation published by Lancaster et al.1,2 Cerebral organoid formation is initiated through an intermediate embryoid body (EB) formation step followed by expansion of neuroepithelia. After a period of maturation, organoids generated using this kit feature cortical-like regions with ventricular zone (PAX6+/SOX2+/Ki-67+), outer subventricular zone (Ki-67+/p-Vimentin+), intermediate zone (TBR2+), and cortical plate (CTIP2+/MAP2+/TBR1+). For extended periods of organoid culture (> 40 days), the components required for organoid maturation can be purchased as the STEMdiff™ Cerebral Organoid Maturation Kit (Catalog #08571).